Background: Resolution estimation is the main evaluation criteria for the\nreconstruction of macromolecular 3D structure in the field of cryoelectron microscopy\n(cryo-EM). At present, there are many methods to evaluate the 3D resolution for\nreconstructed macromolecular structures from Single Particle Analysis (SPA) in cryo-EM\nand subtomogram averaging (SA) in electron cryotomography (cryo-ET). As global\nmethods, they measure the resolution of the structure as a whole, but they are\ninaccurate in detecting subtle local changes of reconstruction. In order to detect the\nsubtle changes of reconstruction of SPA and SA, a few local resolution methods are\nproposed. The mainstream local resolution evaluation methods are based on local\nFourier shell correlation (FSC), which is computationally intensive. However, the\nexisting resolution evaluation methods are based on multi-threading implementation\non a single computer with very poor scalability.
Loading....